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University of North Carolina at Chapel Hill

Dowen RH, Ahmed S. Maternal Inheritance: Longevity Programs Nourish Progeny via Yolk. Curr Biol 2019 Aug 5;29(15):R748-R751. PMID: 31386852

 

 

Dowen, RH. CEH-60/PBX and UNC-62/MEIS coordinate a metabolic switch that supports reproduction in C. elegans. Dev Cell 2019 Apr 22;49(2):235-50. PMID: 30956009

The molecular basis of how animals integrate metabolic, developmental, and environmental information before committing resources to reproduction is an unresolved issue in developmental biology. In C. elegans, adult animals reallocate fat stores from intestinal cells to the germline via low-density lipoprotein (LDL)-like particles to promote embryogenesis. Here, I demonstrate that two conserved homeodomain transcription factors, CEH-60/PBX and UNC-62/MEIS, coordinate a transcriptional network that supports reproduction while suppressing longevity and stress response pathways. The CEH-60:UNC-62 heterodimer serves an unanticipated dual function in intestinal nuclei by directly activating the expression of lipoprotein genes while directly repressing stress-responsive genes. Consequently, ceh-60 mutants display fat storage defects, a dramatic lifespan extension, and hyper-activation of innate immunity genes. Finally, CEH-60 associates with PQM-1 at the DAF-16-associated element within the promoters of stress-responsive genes to control gene expression. Thus, CEH-60 governs an elaborate transcriptional network that balances stress responses and longevity against reproduction during developmental transitions.

Massachusetts General Hospital

Massachusetts General Hospital

Dowen, RH, Breen, PC, Tullius, T, Conery, AL, Ruvkun, G. A microRNA program in the C. elegans hypodermis couples to intestinal mTORC2/PQM-1 signaling to modulate fat transport. Genes Dev 2016 Jul 1;30(13):1515-28. PMID: 27401555

Cheesman, HK, Feinbaum, RL, Thekkiniath, J, Dowen, RH, Conery, AL, Pukkila-Worley, R. Aberrant Activation of p38 MAP Kinase-Dependent Innate Immune Responses Is Toxic to Caenorhabditis elegans. G3 2016 Jan 27;6(3):541-9. PMID: 26818074

Riedel, CG, Dowen, RH, Lourenco, GF, Kirienko, NV, Heimbucher, T, West, JA, Bowman, SK, Kingston, RE, Dillin, A, Asara, JM, and Ruvkun, G. DAF-16 employs the chromatin remodeller SWI/SNF to promote stress resistance and longevity. Nat Cell Biol 2013 May;15(5):491-501. PMID: 23604319

Montgomery, TA, Rim, YS, Zhang, C, Dowen, RH, Phillips, CM, Fischer, SE, and Ruvkun, G. PIWI associated siRNAs and piRNAs specifically require the Caenorhabditis elegans HEN1 ortholog henn-1. PLoS Genet 2012 Apr 19;8(4). PMID: 22536158

 

University of California San Diego

Qi, D, Dubiella, U, Kim, SH, Sloss, DI, Dowen, RH, Dixon, JE, and Innes, RW. Recognition of the protein kinase AVRPPHB SUSCEPTIBLE1 by the disease resistance protein RESISTANCE TO PSEUDOMONAS SYRINGAE5 is dependent on s-acylation and an exposed loop in AVRPPHB SUSCEPTIBLE1. Plant Physiol 2014 Jan;164(1):340-51. PMID: 24225654

Dowen, RH, Pelizzola, M, Schmitz, RJ, Lister, R, Dowen, JM, Nery, JR, Dixon, JE, and Ecker, JR. Widespread dynamic DNA methylation in response to biotic stress. Proc Natl Acad Sci USA 2012 Aug 7;109(32):E2183-91. PMID: 22733782

Lister, R*, Pelizzola, M*, Dowen, RH, Hawkins, RD, Hon, G, Tonti-Filippini, J, Nery, JR, Lee, L, Ye, Z, Ngo, Q, Edsall, L, Antosiewicz-Bourget, J, Stewart, R, Ruotti, V, Millar, AH, Thomson, JA, Ren, B, and Ecker, JR. Human DNA methylomes at base resolution show widespread epigenomic differences. Nature 2009 Nov 19;462(7271):315-322. *Equal contribution. PMID: 19829295

Dowen, RH, Engel, JL, Shao, F, Ecker, JR, and Dixon, JE. A Family of Bacterial Cysteine Protease Type III effectors utilize acylation-dependent and independent strategies to localize to plasma membranes. J Biol Chem 2009 Jun 5;284(23):15867-79. PMID: 19346252

Gentry, MS, Dowen, RH, Worby, CA, Mattoo, S, Ecker, JR, and Dixon, JE. The phosphatase laforin crosses evolutionary boundaries and links carbohydrate metabolism to neuronal disease. J Cell Biol 2007 Jul 23;178(3):477-88. PMID: 17646401

Digicaylioglu, M, Kaul, M, Fletcher, L, Dowen, R, and Lipton, SA. Erythropoietin protects cerebrocortical neurons from HIV-1/gp120-induced damage. Neuroreport 2004 Apr 9;15(5):761-3. PMID: 15073510